Repairing renal injuries with cyanoacrylates

-
In abdominal trauma, renal lesions are relatively common (30.3%) and in approximately 4% of the cases of renal trauma, a complete nephrectomy is necessary. The search for a fast, effective hemostatic method has led us to evaluate a new elastic cyanoacrylate in renal penetrating trauma injuries. The experiment was divided into four groups: The Control Group, Time 1 (after 48 hours of healing), Time 2 (after 6 days) and Time 3 (18 days after surgery). In the Control Group, a laparotomy was performed and closed in layers with a conventional suture. In Times 1, 2 and 3, a laparotomy was carried out and a penetrating traumatic injury was created in the superior and inferior poles of each of the kidneys, making a total of four lesions per animal with a biopsy punch 0.8 cm in diameter at a depth of 3 mm. In each lesson, a different treatment was applied: One with a purified collagen sponge (Gelita-Spon®), another sponge with fibrinogen and thrombin (TachoSil®), one with the elastic cyanoacrylate adhesive, and one wound was left to heal by itself. The Control Group animals were euthanized when they reached 400 g in weight, and those from the Times 1, 2 and 3 groups at the time indicated.

Picture3.png
After taking samples, a full histopathologic study was carried out, involving staining with hematoxylin-eosin, Masson’s trichrome, Schiff (PAS) and the immunohistochemical markers CD31 (angiogenesis) and CD68 (macrophages). Measurements were also taken in microns (μm) for the distance between the edges of the lesion.

The proteomic determinations indicate that applying the cyanoacrylate with inflammatory cytokines led to an expression that corresponds to a normal inflammatory process. For the metalloproteinases, no differences were observed in the concentration of proteolytic enzymes in the lesions treated with adhesive compared to the other treatments.

The histopathology reveals that the inflammatory response observed is similar in all the treatments studied. There are no significant differences between the lesions where cyanoacrylate adhesive was applied and the other treatments as regards the distance between the edges of the lesion and the size of the area marked by CD31 and CD68.

Cyanoacrylate has proven to be a fast, efficient method for achieving hemostasis in renal penetrating injury. Furthermore, the tests have not detected differences with the other treatments used. However, one should keep in mind that it was the treatment that caused the most abdominal adhesions. Despite this, it could be a new alternative technique.

Author: Dolores Cejalvo-Lapeña, Universidad Católica de Valencia, Spain

International Conference on Wound Care, Tissue Repair and Regenerative MedicineDate & venue: June 14-15, 2018 | London, UKWebsite: https://goo.gl/eLcFyZ

Comments

Post a Comment

Popular posts from this blog

Clinical Trials

-
Image
Clinical trials are a critical component of the effort to develop new ways to prevent, detect, diagnose, and treat cancer  and other diseases. "Some people think that clinical trials are the last resort," says Jack Whelan of Andover, Mass., who was diagnosed with a rare form of non-Hodgkin lymphoma in 2007. "I am a survivor of a rare not-yet-curable cancer. Forward-looking science, what is available in clinical trials, was the best choice for me." For researchers and clinicians, trials are critical to determining the potential benefits and safety of investigational cancer therapies  or for understanding if an existing medication could be useful for other types of cancer . Clinical trials are the last step before treatments are made widely available. They come after the completion of basic lab research and testing. Clinical testing in patients is key to understanding how a particular therapy will perform and what kinds of side effects may occur. Clinical tr
Read more

Solar Greenhouse

-
Image
A greenhouse  is a structure secured with a transparent material (glass or plastic) that goes about as a solar gatherer and uses solar brilliant energy to develop plants. It has heating, cooling and ventilating gadgets  for controlling the temperature inside the greenhouse. Solar radiations can go through the greenhouse coating, yet the warm radiations produced by the items inside the greenhouse can't escape through the coated surface. Subsequently, the radiations  get caught inside the greenhouse and result in an expansion in temperature. As the greenhouse structure has a shut limit, the air inside the green­house gets advanced with CO2 as there is no blending of the greenhouse air  with the encompassing air. Further, there is diminished dampness misfortune because of limited tran­spiration. Every one of these highlights helps to maintain plant development for the duration of the day and additionally amid the night and throughout the entire year. To know more: ht
Read more

What Is Cancer Research?

-
Image
Cancer research  transforms and saves lives. The goal of studying cancer is to develop safe and effective methods to prevent, detect, diagnose, treat, and, ultimately, cure the many diseases we call  cancer . The better we understand these diseases, the more progress we will make toward diminishing the tremendous human and economic tolls of  cancer .  Research has helped us accumulate extensive knowledge about the biological processes involved in  cancer  onset, growth, and spread in the body. Those discoveries have led to more effective and targeted treatments and prevention strategies. Breakthroughs in prevention, early detection, screening, diagnosis, and treatment are often the result of research and discoveries made by scientists in a wide array of disciplines over decades and even generations. Ultimately , cancer research  requires partnerships and collaborations involving researchers, clinicians, patients, and others to translate yesterday's discoveries into today&#
Read more