Two in vitro morphogenesis pathways leading to whole plant regeneration

The early expression of WUSCHEL, an organ identity gene, is a marker in tobacco and Beta palonga during the onset of in vitro shoot morphogenesis

Author-Gaurab Gangopadhyay, Division of Plant Biology, Bose Institute (Main Campus), India
The two in vitro morphogenesis pathways leading to whole plant regeneration involve either shoot organogenesis followed by root organogenesis or somatic embryogenesis. Both developmental pathways can occur either directly without an intermediate callus stage, or indirectly following an unorganized callus stage. Reports of involvement of WUS gene concerning in vitro shoot organogenesis are very scanty, particularly in the non-model plant systems, i.e., plants other than Arabidopsis. We have studied the role of growth regulators behind in vitro shoot organogenesis and somatic embryogenesis in two plant systems, viz. tobacco (Nicotiana tabacum L. var. Jayasri) and Beta palonga R.K.Basu & K.K.Mukh, a model, and a non-model plant system respectively.

We have also correlated the phenomena of dedifferentiation with the relative expression of WUS (WUSCHEL) gene in a time-dependent manner. The results indicated that early WUS gene expression is a definite marker for in vitro shoot organogenesis in tobacco and Beta both in direct and indirect modes of regeneration. Additionally, we have performed a comparative homology modeling and in the silico structural analysis of WUSCHEL proteins of B. palonga, B. vulgaris, and Arabidopsis to find out the commonality of the ligand binding site. The amino acids of the binding sites were identical (Arginine, Tryptophan, Proline, Asparagine, and Tyrosine) in the three materials under study; except two additional amino acids (Isoleucine and Alanine) in B. vulgaris.

Conference- International Conference on Plant ScienceDates- August 6-8 2018Venue- Vienna, AustriaWebsite- https://bit.ly/2JvW4lR

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